Nanoinjectors for the specifics scanning probe microscope with the preparative and analytical posibilities for the medical and biological applications (“Nanoinjector”)

The purpose of this project is the implementation of the application specific probe instruments, combined with the ultra precision batchmeter for the analytical device on the basis of the scanning probe microscope with the functions of the microinjectors for an investigation of processes of the cellula systems vital functions, microorganismes and tissues, the investigations of the biological structures reactions on the changes of the chemical composition, temperature and other parameters of an environment, the investigations of the structure of DNA, RNA, of the large molecules with the possibility their local modification by means of tenso-, electro- or chemical influences.

Implemented device must to be:
The cartridge with the specific microprobe head – injectors with the functions of micropumps and simultaneously of probes for the scanning probe microscopes. These cartridges represents the micromechanical actuating elements, capable simultaneously to realize both the analysis and the preparative influences on the cells, tissues, separate molecules.
The device with such possibilities will fulfill the functions of transport with the reversible movement within the limits of macroscopic field with the precision more better of the maximal scan dimension (on a level 1 mm by the scan dimension 50 x 50 μm).
· provide the possibility of the surface control on the object glasses measuring 25 x 20 x 2 mm3;
· be in a position to research the phase – interfaces solid body – liquid with the controlled parameters;
· using as liquid cell an incompletely sealed Petri dish with the controlled atmosphere;
· have the possibility both the full change and an alteration of the liquid composition;
· have in their list of single order parts the controlled micromechanical probe injectors for the directed input to the selected location of the investigated object of an assigned dose of the required substance;
· have the properties of the multimode scanning probe microscope with the following operating conditions:

- contact and lateral microscopy modes; mode of the resonance and modulation microscopy; possibility of the registering of the module, the amplitude projections on the vector of the signal excitated the oscillation; possibility of the registering and support of the oscillation phase; possibility of the registering of the fractional harmonics with the highest frequency up to 1.8 MHz; - magnetoforce mode and the mode of the registering of the electrical forces; current mode with the simultaneously registering of the surface topographical characteristics; adhesive mode; - thermomode with the possibility to measure of the thermal fields around on the object surface with the nanometer spatial resolution and relative resolution according to temperature up to 0.02°C in a temperature range - 40°C + 140°C; - nanolitography unit mode with a possibility of an implementaion of the modulation tensoinduced modification of the surface.

The realization of such cartridges will provide the possibilities expansion of the scanning probe microscope and to raise the researches level by programs “Technology of the living systems” by such major directions, as “Genom of Man”, “Priority directions of a genetics”, “Biological Varieties”, “Newest methods of the bioengineering” and “Provision means of the researches by physical and chemical biology and biotechnology”.

Actuality
The development of the methods of gene – engineering, researches of the intermediate stages of the viral infecting of cells, transdermal delivery of the medicinal preparations, an identification of the microorganisms and viruses, the researches in the field of nervous tissues are require the realization of the instruments, capable to make the investigations of the relief surfaces, which are the cells and the cuts of tissues of the organisms under adequate conditions of their existence (liquid environments) with a possibility of the directed influence on such systems. At present the level of the biological and medical sciences approached to the boader, when without an implementation of such type device the investigations are considerably delayed and an information is received only indirectly.
The interest of the molecular biologists and physiciancs is to considerable extent determined by a hope on a principal realization of such operations, that, unfortunately, did not achieve in the full volume on the devices, available at present.

Novelty
At present the offered devices are absent. On the construction of such devices it is possible the patenting.