The name of the project The development of the low temperature processes of the forming of the biocompatible structures and the researches of the physical and chemical, biomedical properties of the modifying surfaces of such structures for the use in the transplantation of the artificial organs
The contents of works

The modeling of the properties of the natural tissues of the man organism is an one from perspective directions for the implementation of the biomaterials for the implants, substituted the functions of the vitally important organs.
The project is directed on the solution of the basic task in the field of the development of the artificial or bioartificial (hybrid) organs, connected with theoretical an experimental research of the mechanism of an interaction with blood and the physiological solution of the man of an alien surface, modeling the hydrophilic and hydrophobic nanostructure of an endothelium of the blood vessels of the man, the cells membrans of a blood and an albumen. The basic task of the project is the revealing of the quantitative interactions between the physical and chemical and biological properties of the hydrophilic and hydrophobic nanostructures, forming on the surface of the solid states. For the realization of the such structures will developed and improved the original low methods of the chemical and physical (plasma chemical deposition, deposition from an ion plasma flow, chemical vapor deposition with the using of the photon stimulation) surface immobilization of the hydrophilic (C-, OH-, NH-, NH_2-, SO_3-) and hydrophobic (F-, CH_3-) functional groups. As varied parameters of the forming nanostructures will be taken the quantity, nature and ratio of the vaccinated functional groups, the morphology of the surface, the thickness of the modifying layer. As the substrates for the forming of the biocompatible structures was selected the materials of the crystalline structures (quartz, semiconductors) and noncrystal structures (medical polymers, low density polyethylene, polyethylene-teraphthalate, polyurethane "Vitur", polyhydroxyethylmetacrylat), that will allow to realize the maximum technological possibilities of the used methods and the study of the initial stage of an interaction of the active centers of the nanostructures with the blood components and the physiological solution of the man on the molecular and the cell levels under conditions in vitro.
The methods of the scanning tunnel microscopy, scanning electron microscopy, Raman-, infared-, and X - ray photoelectric spectroscopy, the measurement of the contact angles of the wetting on the wafer and the standard liquids with the subsequent calculation of the interphase surface energy, their polar and dispersion components.
The biomedical properties of the investigated materials will valued by means of following methods: a fluorescence of the full internal reflection for the measurement of the kinetics of an adsorption of the basic albumen of the blood plasma (albumen, fibrinogen, globulin) in continuous mode and in a real time from the model solutions, serum, blood plasma; the radiosotope method for the determination of the adhesive trombocite masse; the methods of the light diffusion for the research of the degree of the trombocites activation induced by the surface (the distribution on the size and the morphology, aggregation, the detachment of the trombocite factors); the photocolometer method of the kinetic titration for the registering of the system activation of the complements - the system of the humoral immunological answer.
The complex use of the wide spectrum of the up - to- date physical and biological research methods in combination with the methods of the mathematical modeling allow to determine an interaction between the structural, energy and biological properties of the structures.
Expected results
The novelty and priority of the expected results of an operation outstrip the known foreign researches by the following positions: the methods of the modifying of the surface and the realization of the biocompatible nanostructures; the formulating and the experimental basing of the necessity and the sufficient physical and chemical and biological properties of the haemocompatible interfaces. It gives the basis to hope for reception of new knowledge about the mechanism.